Eficácia da suplementação oral com 1, 3-1, 6 betaglucano proveniente de Saccharomyces cerevisiae no controle da mastite bovina / [Efficiency of oral supplementation of 1. 3-1. 6 beta-glucan from saccharomyces cerevisiae on the control of bovine mastitis]

A mastite bovina, uma das principais doenças do rebanho leiteiro, caracteriza-se por um processo inflamatório no úbere. A inviabilidade econômica, o impacto ambiental negativo e os resíduos antimicrobianos têm estimulado a pesquisa de outros tratamentos alternativos para a prevenção e o tratamento de doenças na bovinocultura leiteira. O betaglucano é um agente imunomodulador com potencial ação preventiva para doenças infecciosas, inclusive a mastite. Este estudo teve como objetivo avaliar a eficácia do uso do betaglucano, por meio de administração oral, em animais em lactação. Foram utilizadas 20 vacas lactantes, distribuídas em dois grupos, um controle e um tratamento, com 10 animais em cada grupo. O grupo tratamento recebeu 5g/dia, durante 60 dias, de 1,3-1,6 betaglucano isolado da parede celular de Saccharomyces cerevisiae diluído em ração após a ordenha, enquanto o grupo controle recebia somente a ração. Foram realizados os testes de California Mastitis Test (CMT), contagem de células somáticas (CCS), produção de leite e percentual de gordura e proteína no leite. Não houve diferença estatisticamente significativa entre os grupos quanto à CCS, ao CMT, à composição do leite ou produção. Não se observou, portanto, eficácia do uso do betaglucano purificado, administrado por via oral, no controle e na prevenção da mastite em vacas leiteiras, quando comparadas com o grupo controle. Atribuem-se esses resultados, principalmente, à degradação ruminal do produto testado. Sugerem-se, portanto, mais pesquisas utilizando o 1,3-1,6 betaglucano purificado de parede de S. cerevisiae por outras vias de administração, tais como intramamária e subcutânea.(AU)

Bovine mastitis, one of the main diseases of dairy herds, is characterized by an inflammatory process in the udder. The economic and environmental impacts, as well as the residues of antimicrobial drugs have stimulated the research of novel alternative treatments for the prevention and treatment of diseases in dairy production cows. The beta-glucan is an immunomodulator agent, with potential preventive action for infectious diseases, including mastitis. This study aimed to assess the effectiveness of orally administered beta-glucan in lactating cows. 20 lactating cows were used, distributed into two groups, one control and one treatment, with 10 cows in each group. The treatment group received 5g of 1.3-1.6 betaglucan daily for 60 days, isolated from the cell-wall of Saccharomyces cerevisiae diluted into a grain meal, whereas the animals in the control group received only the ration. The California Mastitis Test (CMT), Somatic Cells Counting (SCC), daily production and assessments of fat and protein content in milk were done. There was no statistically significant difference between the groups concerning subclinical mastitis detected by CMT, SCC, milk production and composition regarding protein and fat content. It was not observed, therefore, the effectiveness of the use of purified beta-glucan orally administered on the control or prevention of mastitis in dairy cows. The results are attributed to the ruminal degradation of the product tested. It is, therefore, suggested that more research should be conducted using the 1.3-1.6 beta-glucan purified from the cell wall of S. cerevisiae by other administration means and ruminal protection technologies for the isolated beta-glucan.(AU)

Role of the consumption of fucoidans and beta-glucans on human health: an update of the literature / Rol del consumo de fucoidanos y betaglucanos en la salud humana: artículo de actualización

ABSTRACT Bioactive compounds are substances present in low doses in foods mostly from the plant kingdom. Their consumption can influence physiological or cellular processes and has a beneficial effect on health. Fucoidans and beta-glucans are bioactive compounds that share the characteristic of being carbohydrates of the polysaccharide type, specifically of the soluble fiber kind. These compounds can be found in foods available in Chile and part of the Chilean diet; foods include, brown algae and some cereals. The concentration of these compounds in foods is variable, and depends on factors like season, cultivation, germination, and method of preparation and conservation. The current literature shows the potential beneficial effects of fucoidan and beta-glucans compounds in human health, which include: anticoagulant, immuno-modulator and antidiabetic and immunomodulating. The effects depend, among other factors, on their bioavailability and molecular weight. The objective of this review was to describe the potential beneficial effects of these bioactive compounds, analyze their characteristics and properties, and provide consumption recommendations that may lead to achieving the expected beneficial effects. To that end, relevant and recent scientific literature was analyzed.

RESUMEN Los compuestos bioactivos corresponden a aquellos elementos presentes en los alimentos, que influyen en procesos fisiológicos o celulares, cuyo consumo produce un efecto beneficioso para la salud, encontrándose en alimentos principalmente del reino vegetal y cuyas concentraciones en los alimentos son en mínimas dosis. Los fucoidanos y los beta-glucanos, son compuestos bioactivos que comparten la característica de ser un carbohidrato, de tipo polisacárido, específicamente fibra de tipo soluble, encontrándose presente en alimentos disponibles en nuestro país y que son parte de nuestra dieta, como las algas pardas y algunos cereales. La concentración de estos compuestos en los alimentos, es fluctuante y depende de factores como la estacionalidad, cultivo, germinación, método de preparación y conservación. En la bibliografía actual, se evidencian los potenciales efectos beneficiosos de fucanos y beta-glucanos para la salud humana como anticoagulante, antidiabético e inmunomodulador, la intensidad de estos efectos dependen de su biodisponibilidad y del peso molecular. El objetivo de esta revisión fué profundizar respecto a los potenciales efectos beneficiosos de estos compuestos bioactivos, analizar sus características, propiedades, además de relacionar una recomendación de consumo que permita alcanzar los efectos beneficiosos esperados. Para ello se analizó la bibliografía científica atingente, relevante y reciente.

ß-Glucanase Addition in Brewing Malt Produced by Reduced Time of Germination

Abstract The β-Glucans content has straight influence on the quality of malt and beer, mainly during the filtration step. Barley presenting high β-Glucan content demands longer germination time at malting. The application of commercial β-Glucanase is an alternative to accelerate the process and preserve the quality of malt. This work aimed to evaluate the effect of commercial β-Glucanase addition in malt produced within reduced germination time (64 h). Micro-malting was conducted with BRS-Caue and Elis barley cultivars at germination time 64 h and 96 h. The β-Glucanase concentration applied were 0, 25, 50 and 100 mg.kg-1. Barley, malt and wort samples were analyzed to check their physical-chemical features. Beers were produced with BRS-Caue malt and the physical-chemical and sensory attributes were analyzed. The commercial enzyme addition in BRS-Caue and Elis (64 h), at concentration 25 and 50 mg.kg-1, resulted in wort presenting proper β-Glucan content (≤ 178 mg.L-1). The beer produced with malt germinated for 64 h and added with 50 mg.kg-1 of β-glucanase was the one showing the largest number of physical-chemical and sensory parameters similar to the beer made with malt germinated for 96 h (conventional process). Commercial β-glucanase application in malt allowed accelerating the malting process without affecting the quality of the malt for beer production.

Disease resistance of pacu Piaractus mesopotamicus (Holmberg, 1887) fed with β-glucan / Resistência à doença em pacu, Piaractus mesopotamicus (Holmberg, 1887), alimentados com β-glucana

Effects of β-glucan on innate immune responses and survival were studied in pacu experimentally infected with Aeromonas hydrophila. Fish fed diets containing 0, 0.1% and 1% β-glucan were injected with A. hydrophila. β-glucan enhanced fish survival in both treated groups (26.7% and 21.2% of the control, respectively). Leukocyte respiratory burst and alternative complement pathway activities were elevated after bacterial challenge regardless the β-glucan concentration. Lysozyme activity was higher after infection and showed a gradual increase as β-glucan concentration increased. A significant elevation in WBC count was observed either after bacterial challenge or by influence of β-glucan separately. The same response was observed in the number of thrombocytes, lymphocytes, eosinophils, LG-PAS positive cell and monocytes. It can be concluded that feeding pacu with β-glucan can increase protection against A. hydrophila, due to changes in non-specific immune responses.

Os efeitos da β-glucana sobre as respostas imunes inatas e a sobrevivência foram estudados em pacu experimentalmente infectado com Aeromonas hydrophila. Peixes alimentados com dietas contendo 0,1% e 1% de β-glucana foram injetados com 1 × 108 CFU de A. hydrophila após 7 dias de alimentação. A sobrevivência de peixes foi maior nos dois grupos tratados em comparação ao grupo controle (26,7% e 21,2%, respectivamente). A atividade respiratória de leucócitos e a atividade hemolítica do complemento – via alternativa estavam elevadas após desafio bacteriano independentemente da concentração de β-glucana. A atividade de lisozima foi maior após a infecção e mostrou um aumento gradual de acordo com a concentração do imunoestimulante. Observou-se um aumento significativo na contagem de leucócitos totais após o desafio bacteriano e influência de β-glucana. A mesma resposta foi observada para trombócitos, linfócitos, eosinófilos, leucócito PAS positivo e monócitos. Com exceção de neutrófilos, que diminuíram frente ao mais alto nível do imunoestimulante e não se alteraram após a infecção, as outras células aumentaram após a exposição à A. hydrophila. A β-glucana não afetou os níveis de proteína total do soro, que aumentaram após o desafio bacteriano. Conclui-se que a administração de β-glucana em pacu pode aumentar a proteção contra A. hydrophila, por alterações nas respostas imunes de não-específicas.

Respostas fisiológicas e de desempenho de leitões suplementados com B-glucanos e desafiados imunologicamente / Phisiologial responses and growth performance of immune challenged piglets supplemented with B-glucans

O estudo buscou avaliar as respostas fisiológicas e de desempenho à adição de diferentes níveis de β-glucanos na dieta de leitões recém-desmamados. Foram utilizados 30 machos de linhagem comercial, com 34 dias de idade e peso de 10,9±0,63kg, alojados em gaiolas individuais, durante 14 dias. Os tratamentos variaram somente nos níveis de β-glucanos que foram de 35, 70, 140 e 280g/T. No final desse período, em metade dos animais foram injetados 2,7mg de lipopolissacarídeo (LPS) em 1mL de solução (PBS)/leitão, e nos demais 1mL/leitão de PBS. Foram avaliadas temperatura retal (TR), frequência respiratória (FR), perfil bioquímico sanguíneo e sinais clínicos aos 30, 90, 120 e 390 minutos seguintes à injeção. As respostas de desempenho não foram afetadas pelos níveis de β-glucanos. Foi observado o aumento de TR, tanto em função do LPS como em função da hora em que a temperatura foi medida. No entanto, os animais LPS-injetados, que receberam 280g/T de β-glucanos, tiveram a mesma TR daqueles LPS-não injetados. Os sinais clínicos foram compatíveis com um quadro de inflamação aguda e foram observados, nos animais LPS-injetados, vômito, prostração e diarreia, sendo que a inclusão de β-glucanos não conseguiu reverter esses sintomas. O uso de β-glucanos por 14 dias na dieta de leitões recém-desmamados mostrou um efeito anti-inflamatório em situação de desafio agudo. Para reverter o desafio imunológico utilizado, o nível de 280g/T de β-glucanos mostrou-se o mais recomendado.

This study was done to evaluate immunological responses and performance of weaning piglets receiving diets with different levels of β-glucans. Thirty males from a commercial line with 34 days of age and 10.9±0,6kg initial weight were housed in individual metabolic cages during 14 days. The treatments only differed in β-glucans levels: 35; 70; 140 and 280g/T. On the 14th day, half of the pigs were inoculated with 2.7mg LPS (lipopolysaccharide)/mL of solution (PBS)/pig and the other half received 1mL PBS/pig. Rectal temperature (RT), respiratory frequency (RF), biochemical profile and animal behavior (120 and 390 minutes after LPS inoculation) were evaluated. As the experiment was conducted, performance was not affected by β-glucans. An RT increase was observed due to LPS and time of temperature measurement. LPS-injected animals receiving 280g/T of β-glucans had the same RT as the LPS- non injected group. Clinical signs were near the expectation for acute inflammation. LPS-injected presented prostration, diarrhea and vomit and the β-glucans did not reverse this situation. The use of β-glucans in weaning pigs for fourteen days showed an anti-inflammatory action. To revert the immunologic challenge, the level of 280g/T of β-glucans was the most recommended.

Fibras na dieta: tendências atuais e benefícios à saúde na síndrome metabólica e no diabetes melito tipo 2: [revisão] / Dietary fibers: current trends and health benefits in the metabolic syndrome and type 2 diabetes: [review]

As fibras dietéticas parecem contribuir tanto na prevenção quanto no tratamento do diabetes melito tipo 2 (DMT2). Em estudos epidemiológicos a ingestão de fibras insolúveis, e não de fibras solúveis, tem sido inversamente associada à incidência do DMT2. Por outro lado, em estudos pós-prandiais, refeições contendo quantidades suficientes de β-glucano, psyllium, ou goma-guar diminuíram as respostas da insulina e da glicose, tanto em indivíduos saudáveis como em pacientes com DMT2. Dietas enriquecidas com quantidade suficiente de fibras solúveis também parecem melhorar o controle glicêmico de uma forma geral no DMT2. As fibras insolúveis têm pouco efeito sobre as respostas pós-prandiais de insulina e glicose. As fibras da dieta aumentam a saciedade. Em alguns estudos, as fibras solúveis têm se associado com um menor aumento de peso corporal ao longo do tempo. Evidências limitadas, a partir de estudos transversais, sugerem uma associação inversa entre o consumo de fibras dos cereais e de grãos integrais e a prevalência de síndrome metabólica. Apesar da escassez de dados sobre estudos de mais longo prazo que foquem especificamente em fibras dietéticas, seguir a recomendação atual de 25 g de fibras ao dia, a partir de uma dieta rica em grãos integrais, frutas e legumes, provavelmente diminuirá o risco para a obesidade, síndrome metabólica e DMT2.

Dietary fiber may contribute to both the prevention and treatment of type 2 diabetes mellitus (T2DM). In epidemiological studies the intake of insoluble fiber, but not the intake of soluble fiber, has been inversely associated with the incidence of T2DM. In contrast, in postprandial studies, meals containing sufficiently quantities of β-glucan, psyllium, or guar gum have decreased insulin and glucose responses in both healthy individuals and patients with T2DM. Diets enriched sufficiently in soluble fiber may also improve overall glycemic control in T2DM. Insoluble fiber has little effect on postprandial insulin and glucose responses. Fiber increases satiety. In some studies, insoluble fiber has been associated with less weight gain over time. Limited cross-sectional evidence suggests an inverse relationship between intake of cereal fiber and whole-grains and the prevalence of the metabolic syndrome. Although long-term data from trials focusing on specifically dietary fiber are lacking, meeting current recommendations for a minimum fiber intake of 25 g/d based on a diet rich in whole grains, fruits and legumes will probably decrease the risk of obesity, the metabolic syndrome and T2DM.

Biomedical Issues of Dietary fiber beta-Glucan

beta-Glucan is a polysaccharide in the form of fiber and the main element of fiber in grains such as barley, oats, yeast and mushrooms. Many studies have examined the efficacy of beta-Glucan in terms of the lipid lowering effects, blood sugar reduction, weight reduction, immune modulator, and anticarcinogenic effect. However, there is no comprehensive review article on the biomedical issues regarding beta-Glucan. The authors searched for systematic reviews and clinical experiments for each relevant topic and reviewed the biomedical effects of beta-Glucan, for the purpose of developing research strategies for the future.